Hans Jansen

Session 1: Ultra-High Throughput Screening (uHTS) at the Pivot Park Screening Centre

The Pivot Park Screening Centre (PPSC) is one of the partners in the European Lead Factory (ELF) and is responsible for all the assay development and screening activities. Targets from public partners are screened at the top-modern, industry-standard European Screening Centre (ESC) at no upfront cost, as funding is provided by the IMI. This unique large-scale experiment, combining open innovation, crowdsourcing and several established pharmaceutical companies, is in its 5th year producing exciting scientific results.
Besides the activities in ELF,  the PPSC supports a wide range of lab automation activities like robotic pipetting (DRC’s, plate preparation), multilabel plate readers, fully automated ELISA’s, High Content Screening, Ion channel assays, Phenotypic screens, Live Cell Imaging and High Throughput Screening (HTS).

This presentation will focus on the HTS operations and the involved robotic infrastructure. As of January 2017, more than 70 HTS campaigns were performed covering a wide range of target classes, including more demanding classes like ion channels and protein-protein interactions. Almost all HTS assays have been successfully miniaturized to 1536 format in order to perform them in a cost-efficient manner. Full screens are typically performed within 2-3 days with one example where the 320,000 library was screened in one single day.

Session 2: Date Tracking, Processing and Evaluation at the Pivot Park Screening Centre

The Pivot Park Screening Centre (PPSC) is one of the partners in the European Lead Factory (ELF) and is responsible for all the assay development and screening activities. Targets from public partners are screened at the top-modern, industry-standard European Screening Centre (ESC) at no upfront cost, as funding is provided by the IMI. This unique large-scale experiment, combining open innovation, crowdsourcing and several established pharmaceutical companies, is in its 5th year producing exciting scientific results.
Besides the activities in ELF,  the PPSC supports a wide range of lab automation activities like robotic pipetting (Dose Response Curves, plate preparation),  fully automated ELISA’s, High Content Screening, Ion channel assays, Phenotypic screens, Live Cell Imaging and High Throughput Screening (HTS).

To support and manage the complete data flow in an uHTS campaign is quite a challenge. Starting with compound transfer from source to assay plates, tracking plates and well information through the whole process, gathering reader files and uploading these into our LIMS (Activity Base). Scientist will evaluate the raw- and calculated data using manual and automatic invalidation rules. The results of a complete screening campaign, which can contain over one million data points, are uploaded into Vortex for understanding, refining and visualizing the data.

This presentation will give an overview which tools and steps are used at PPSC to facilitate this process.

About Hans Jansen

Hans Jansen worked in various roles in pharmaceutical industry for 40 years. In 1996 he was involved in setting up the first robotic screening platform at Organon and had a leading role in bringing automation to the lab. After the closure of the research facilities in Oss in 2011 he was responsible for setting up the Ultra High Throughput System at the Pivot Park Screening Centre and is now responsible for Screening Operations and Lab Automation.

 

 

 

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