Jens Fuchser & Rob van der Heijden & Patrick van Houts

New levels in speed and robustness in MALDI-MS for demanding applications; tissue imaging and high-throughput screening for Drug Discovery

During 25 years of commercial development, MALDI-MS became and remained the method of choice for a range of applications. In particular in proteomics, intact protein analysis  (biopharmaceuticals),  polymer characterization, microbiology (identification) and pathology  (imaging),  MALDI-MS plays an essential and predominant role in present-day  R&D and routine applications. Its popularity is not only based on its excellent technical performance, but certainly also based on its ease of use, speed and robustness. Some modern applications however, demand extraordinary performance in speed and robustness because of the extreme number of spectra to be collected. For example in MALDI imaging a tissue is scanned with the laser and every pixel in the image requires the acquisition of numerous spectra. In high-throughput screening where large libraries of compounds are tested for biological activity, the number of samples runs easily into the millions. The latest generation of MALDI-MS is specifically designed for these heavy duty tasks. In this presentation the technological innovations will be discussed and the  performance will be  illustrated with real-life applications.