Aljoscha Wahl, CSE TU Delft
It takes an MS to observe metabolism
The recent years have delivered amazing analytical tools to observe cells, including small molecules involved in metabolism. We use GC- and LC-MS based analytics to quantify intracellular intermediates which allows to obtain novel insights into the intracellular reaction network, it’s thermodynamic driving forces and putative regulation. Crucial for reliable, quantitative results are three key elements – defined, rapid sampling of the cultivation, unbiased extraction and calibrated MS measurements. Using calibrated internal standards, derived from a labelled cell cultivation corrects for sample processing variations and MS matrix effects. The metabolomics approach and different examples will be discussed.
About dr. Aljoscha Wahl
Dr. Aljoscha Wahl’s group within the Biotechnology department of the faculty of Applied Sciences develops approaches to analyse, model and understand metabolic networks and its regulation. Metabolism is the source of energy and building blocks for life. How cells distribute resources and react to the environment depends on their metabolic capabilities: the stoichiometry of the metabolic network but also its regulation. Especially under dynamic environments, thus basically all environments, metabolic regulation closely links with survival and fitness. Aljoscha Wahl holds a PhD in bioengineering (2006) working on E. coli metabolism, then worked two years with mammalian hosts to produce vaccines (influenza virus) and joined TU Delft in 2008.