Cell Culture Scale-Up Using Rigid-Wall, Single-Use Bioreactors
For cultivation of mammalian cells in biopharmaceutical research and manufacturing single-use technology possesses several advantages to autoclavable material. Bioreactor scalability is critical to streamline the adaptation of culture volumes during process development and manufacturing. We investigated the cell culture scale-up capabilities of Eppendorf BioBLU® Single-Use Vessels of different size ranges. Three different vessel sizes (maximum working volumes of 0.25 L, 3.75 L, and 40 L) were selected to represent an approximately ten-fold scale-up between vessels. Within the presentation, engineering parameters critical for scaling-up cell culture processes are discussed, such as vessel and impeller geometry, tip speed, and the volumetric oxygen transfer coefficient (kLa). Using computational fluid dynamics simulations we determined the power numbers of the BioBLU bioreactors. Based on these data we scaled up a mAb production process in CHO cells from 250 mL to 3.75 L to 40 L by keeping constant P/V values (impeller power consumption per liquid volume) among the differently sized vessels. Similar cell growth curves and mAb production profiles were achieved at all three scales.
In summary, this study demonstrates the excellent scalability of the Eppendorf BioBLU Single-Use Vessels for cell culture.
About Christof Knocke
Christof Knocke is Head of Service at Vaudaux-Eppendorf AG in Switzerland. Dr. Knocke received his Ph.D. in bioprocess engineering at the RWTH Aachen University in Germany. After a short stay at the business development organization of North-Rhine Westphalia, he joint DASGIP GmbH. There he worked for six years as a European bioprocess sales specialist. Since five years Christof is active at Eppendorf Switzerland.