Sander Slingerland, Bruker
Fast and accurate identification of Good, Bad and Ugly microorganisms during quality control of biological processes.
Identification of microorganisms is an important part of any microbiology quality control during biological processes for food and pharmaceutical production.
In a biological process microorganisms can be present that are essential for the process (the good). But there is also the risk of contaminating microorganisms being present that can form a health risk (the bad), or those that will spoil the biological process (the ugly).
Confirming the identity of the microorganisms being present in the biological process is an important step to confirm how the biological process is going and in case of a contaminant it defines how to proceed.
Matrix Assisted Laser Desorption/Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS) has proven to be an method capable of identifying a broad range of bacteria, yeast and fungi in short time. By measuring proteomic fingerprints from cultivated microorganisms and comparing those against a ready-to-go library, it is capable of identifying many microorganisms accurately at species level within minutes.
Having this information in a short amount of time allows for earlier reaction to adapt the biological process and start tracing the source of the contaminations, supporting the activities to prevent economic loss.
An additionally benefit this method provides is that companies can easily establish fit-for-purpose libraries based on their own unique strains, which allows for quick screening of both potential useful micro-organisms or further tracing of local contaminants.